Department of Physics, Philipps-University, Renthof 5, D-35032 Marburg, Germany
The influence of inclusion-induced tension on pore formation is investigated theoretically and experimentally. It is shown that labeling with fluorescent dyes can enhance pore formation and induce leakage of adhering vesicles. By analyzing the pore dynamics it is shown that these effects are more pronounced for smaller vesicles. Experimental data is presented which confirms the theoretical predictions. Finally, the influence of the dynamics on rupture of vesicles is analyzed yielding a complete new picture of the transition to bilayer disks.