Planar Lipid Layers and Hybrid S-Layer/Lipid Architectures Characterized by Surface-Sensitive Scattering

Mathias Lösche

Institute of Experimental Physics, University of Leipzig, Linnéstr. 5, D-04103 Leipzig, Germany




Physicochemical and biotechnological achievements in the design of physiologically active supramolecular assemblies have brought about the quest for their submolecular-level characterization. We employ surface-sensitive scattering techniques for the investigation of planar lipid membranes – such as floating monolayers on aqueous surfaces – to correlate structural, functional and dynamic aspects of biomembrane models. In recent years, the advent of third-generation synchrotron x-ray sources has driven the development of realistic, submolecular-scale chemical models [1,2]. Neutron scattering – with its capability to specifically interrogate particular molecular segments through contrast variation – complements the x-ray techniques in an ideal way. In combination, these techniques have been utilized to characterize the architecture of hybrid S-layer/lipid systems [3–5].