This presentation provides an overview of recent progress in molecular electronics
and green plant photosynthesis. We have demonstrated that the isolated Photosystem
I (PSI) reaction center is a nanoscale molecular diode and photovoltaic device.
One goal of our research is construction of molecular electronic devices from
these nanoscale structures. Progress has been demonstrated by direct electrical
contact of emergent electrons from the PSI reaction center by nanoparticle platinum
precipitation. The stable diode properties of isolated reaction centers combined
with the ability to orient PSI by self-assembly on a planar surface makes this
structure a potential building block for 2-D and possibly 3-D devices. A second
goal of our research is restoration of photoreceptor activity to people who are
blind from retinal degenerations such as age-related macular degeneration or retinitis
pigmentosa. We extracted and purified integral membrane PSI reaction centers from
spinach leaves and measured their open and closed circuit photovoltages. The open
circuit value is at least 1 V whereas the closed circuit value is at least 0.6
V. A quantitative analysis of the known physical properties of PSI reaction centers
and voltage-gated ion channels indicates that PSI may be able to optically trigger
opening of the channels. The cell membrane can be depolarized or hyperpolarized
depending on the orientation of the PSI reaction center in the membrane. PSI-proteoliposomes
will be used as the delivery vehicle. We have successfully inserted PSI reaction
centers into liposome membranes and, using P700 absorption spectroscopy, demonstrated
that the reaction centers retain their functional activity in the liposomes. We
have also obtained microscopic evidence that the liposomes are capable of fusing
with the membranes of retinoblastoma cells.